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tricine polyacrylamide gels  (Thermo Fisher)


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    Thermo Fisher tricine polyacrylamide gels
    Tricine Polyacrylamide Gels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+polyacrylamide+gel/TRIS-Tricine-SDS+running+buffer+(10X)%2C+cathode+buffer%2C+pH+8%2E3/pm42218136-299-8-11
    Average 95 stars, based on 1 article reviews
    tricine polyacrylamide gels - by Bioz Stars, 2026-09
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    Related Articles

    other:

    Article Title: Repeated cannabidiol treatment affects neuroplasticity and endocannabinoid signaling in the prefrontal cortex of the Flinders Sensitive Line (FSL) rat model of depression.
    Article Snippet: Delayed therapeutic responses and limited efficacy are the main challenges of existing antidepressant drugs, thereby incentivizing the search for new potential treatments.. Cannabidiol (CBD), non-psychotomimetic component of cannabis, has shown promising antidepressant effects in different rodent models, but its mechanism of action remains unclear.. Herein, we investigated the antidepressant-like effects of repeated CBD treatment on behavior, neuroplasticity markers and lipidomic profile in the prefrontal cortex (PFC) of Flinders Sensitive Line (FSL), a genetic animal model of depression, and their control counterparts Flinders Resistant Line (FRL) rats.

    Article Title: The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.
    Article Snippet: Gels were stainedwith Imperial Blue Stain (Thermo Scientific, 24615).

    Western Blot:

    Article Title: Focal adhesions are controlled by microtubules through local contractility regulation
    Article Snippet: .. Cells were lysed directly in Laemmli buffer for Western blot (Tris–HCl pH 6.8 0.12 M, glycerol 10%, sodium dodecyl sulfate 5%, β-mercaptoethanol 2.5%, bromophenol blue 0.005%) and extracted proteins were separated by SDS–PAGE in 4–20% SDS–polyacrylamide gel (Thermo Fisher Scientific) and transferred to polyvinylidenedifluoride membranes (Bio-Rad) at 75 V for 2 h. Subsequently, the polyvinylidenedifluoride membranes were blocked for 1 h with 5% bovine serum albumin (BSA, Sigma-Aldrich), then incubated overnight at 4 °C with appropriate antibodies: anti-GEF-H1 (Cell Signaling, cat. no. 4145, dilution 1:1000); anti-α-tubulin (Sigma-Aldrich, cat. no. T6199, dilution 1:3000); anti-GAPDH (Santa Cruz Biotechnology, cat. no. sc-32233, dilution 1:3000); anti-APC (Thermo Fisher Scientific, cat. no. A5-35188, dilution 1:1000); anti-ARP2 (Cell Signaling, cat. no. #3128, dilution 1:1000); anti-Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) (Cell Signaling, cat. no. #2605, dilution 1:2000); anti-acetyl-α-Tubulin (Lys40) (Cell Signaling, cat. no. #3971, 1:1000); anti-αTAT1 (Thermo Fisher Scientific, cat. no. PA5-112992, dilution 1:1000); anti-cleaved Caspase-3 (Cell Signaling Technology, cat. no. #9661, 1:1000); anti-mApple antibody (St. John’s Laboratory cat. no. STJ140269, 1:1000), anti-mEmerald antibody (St. John’s Laboratory cat. no. STJ140226, 1:1000), anti-Phospho-FAK (Y397) (Cell Signaling, cat. no. #3283, 1:1000). .. The membranes were washed three times (10 min each) and probed by incubation for 1 h with the appropriate secondary antibodies conjugated with horseradish peroxidase (Bio-Rad).

    Article Title: Focal adhesions are controlled by microtubules through local contractility regulation.
    Article Snippet: .. Cells were lysed directly in Laemmli buffer for Western blot (Tris–HCl pH 6.8 0.12 M, glycerol 10%, sodium dodecyl sulfate 5%, © The Author(s) The EMBO Journal Volume 43 | Issue 13 | July 2024 | 2715 – 2732 2727 D ow nloaded from https://w w w .em bopress.org on M ay 18, 2025 from IP 2400:1a00:b030:4666:489f:28bf:8e13:7fd1. β-mercaptoethanol 2.5%, bromophenol blue 0.005%) and extracted proteins were separated by SDS–PAGE in 4–20% SDS–polyacrylamide gel (Thermo Fisher Scientific) and transferred to polyvinylidenedifluoride membranes (Bio-Rad) at 75 V for 2 h. Subsequently, the polyvinylidenedifluoride membranes were blocked for 1 h with 5% bovine serum albumin (BSA, SigmaAldrich), then incubated overnight at 4 °C with appropriate antibodies: anti-GEF-H1 (Cell Signaling, cat. no. 4145, dilution 1:1000); anti-α-tubulin (Sigma-Aldrich, cat. no. T6199, dilution 1:3000); anti-GAPDH (Santa Cruz Biotechnology, cat. no. sc32233, dilution 1:3000); anti-APC (Thermo Fisher Scientific, cat. no. A5-35188, dilution 1:1000); anti-ARP2 (Cell Signaling, cat. no. #3128, dilution 1:1000); anti-Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) (Cell Signaling, cat. no. #2605, dilution 1:2000); antiacetyl-α-Tubulin (Lys40) (Cell Signaling, cat. no. #3971, 1:1000); anti-αTAT1 (Thermo Fisher Scientific, cat. no. PA5-112992, dilution 1:1000); anti-cleaved Caspase-3 (Cell Signaling Technology, cat. no. #9661, 1:1000); anti-mApple antibody (St. John’s Laboratory cat. no. STJ140269, 1:1000), anti-mEmerald antibody (St. John’s Laboratory cat. no. STJ140226, 1:1000), anti-PhosphoFAK (Y397) (Cell Signaling, cat. no. #3283, 1:1000). .. The membranes were washed three times (10 min each) and probed by incubation for 1 h with the appropriate secondary antibodies conjugated with horseradish peroxidase (Bio-Rad).

    Article Title: Cancer-associated polybromo-1 bromodomain 4 missense variants variably impact bromodomain ligand binding and cell growth suppression
    Article Snippet: .. The resin was resuspended in 1 × Bolt lithium dodecyl sulfate sample buffer (Invitrogen) and boiled for 5 min. Nuclear lysate input and the samples were loaded onto a 4 to 12% SDS-polyacrylamide gel (Invitrogen) for immunoblotting. ..

    Incubation:

    Article Title: Focal adhesions are controlled by microtubules through local contractility regulation
    Article Snippet: .. Cells were lysed directly in Laemmli buffer for Western blot (Tris–HCl pH 6.8 0.12 M, glycerol 10%, sodium dodecyl sulfate 5%, β-mercaptoethanol 2.5%, bromophenol blue 0.005%) and extracted proteins were separated by SDS–PAGE in 4–20% SDS–polyacrylamide gel (Thermo Fisher Scientific) and transferred to polyvinylidenedifluoride membranes (Bio-Rad) at 75 V for 2 h. Subsequently, the polyvinylidenedifluoride membranes were blocked for 1 h with 5% bovine serum albumin (BSA, Sigma-Aldrich), then incubated overnight at 4 °C with appropriate antibodies: anti-GEF-H1 (Cell Signaling, cat. no. 4145, dilution 1:1000); anti-α-tubulin (Sigma-Aldrich, cat. no. T6199, dilution 1:3000); anti-GAPDH (Santa Cruz Biotechnology, cat. no. sc-32233, dilution 1:3000); anti-APC (Thermo Fisher Scientific, cat. no. A5-35188, dilution 1:1000); anti-ARP2 (Cell Signaling, cat. no. #3128, dilution 1:1000); anti-Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) (Cell Signaling, cat. no. #2605, dilution 1:2000); anti-acetyl-α-Tubulin (Lys40) (Cell Signaling, cat. no. #3971, 1:1000); anti-αTAT1 (Thermo Fisher Scientific, cat. no. PA5-112992, dilution 1:1000); anti-cleaved Caspase-3 (Cell Signaling Technology, cat. no. #9661, 1:1000); anti-mApple antibody (St. John’s Laboratory cat. no. STJ140269, 1:1000), anti-mEmerald antibody (St. John’s Laboratory cat. no. STJ140226, 1:1000), anti-Phospho-FAK (Y397) (Cell Signaling, cat. no. #3283, 1:1000). .. The membranes were washed three times (10 min each) and probed by incubation for 1 h with the appropriate secondary antibodies conjugated with horseradish peroxidase (Bio-Rad).

    Article Title: Focal adhesions are controlled by microtubules through local contractility regulation.
    Article Snippet: .. Cells were lysed directly in Laemmli buffer for Western blot (Tris–HCl pH 6.8 0.12 M, glycerol 10%, sodium dodecyl sulfate 5%, © The Author(s) The EMBO Journal Volume 43 | Issue 13 | July 2024 | 2715 – 2732 2727 D ow nloaded from https://w w w .em bopress.org on M ay 18, 2025 from IP 2400:1a00:b030:4666:489f:28bf:8e13:7fd1. β-mercaptoethanol 2.5%, bromophenol blue 0.005%) and extracted proteins were separated by SDS–PAGE in 4–20% SDS–polyacrylamide gel (Thermo Fisher Scientific) and transferred to polyvinylidenedifluoride membranes (Bio-Rad) at 75 V for 2 h. Subsequently, the polyvinylidenedifluoride membranes were blocked for 1 h with 5% bovine serum albumin (BSA, SigmaAldrich), then incubated overnight at 4 °C with appropriate antibodies: anti-GEF-H1 (Cell Signaling, cat. no. 4145, dilution 1:1000); anti-α-tubulin (Sigma-Aldrich, cat. no. T6199, dilution 1:3000); anti-GAPDH (Santa Cruz Biotechnology, cat. no. sc32233, dilution 1:3000); anti-APC (Thermo Fisher Scientific, cat. no. A5-35188, dilution 1:1000); anti-ARP2 (Cell Signaling, cat. no. #3128, dilution 1:1000); anti-Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) (Cell Signaling, cat. no. #2605, dilution 1:2000); antiacetyl-α-Tubulin (Lys40) (Cell Signaling, cat. no. #3971, 1:1000); anti-αTAT1 (Thermo Fisher Scientific, cat. no. PA5-112992, dilution 1:1000); anti-cleaved Caspase-3 (Cell Signaling Technology, cat. no. #9661, 1:1000); anti-mApple antibody (St. John’s Laboratory cat. no. STJ140269, 1:1000), anti-mEmerald antibody (St. John’s Laboratory cat. no. STJ140226, 1:1000), anti-PhosphoFAK (Y397) (Cell Signaling, cat. no. #3283, 1:1000). .. The membranes were washed three times (10 min each) and probed by incubation for 1 h with the appropriate secondary antibodies conjugated with horseradish peroxidase (Bio-Rad).

    Mass Spectrometry:

    Article Title: The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.
    Article Snippet: .. Mass spectrometry of purified RISCs 10 ng of AGO2 protein from each RISC, as quantified by titration filter binding, was denatured with 4X LDS sample buffer (Invitrogen, NP0007) and run on an SDS-polyacrylamide gel (NuPAGE 4 to 12%, Bis-Tris [Invitrogen, NP0322]) in MOPS buffer (Invitrogen, NP0001), according tomanufacturer’s instructions. .. Gels were stainedwith Imperial Blue Stain (Thermo Scientific, 24615).

    Purification:

    Article Title: The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.
    Article Snippet: .. Mass spectrometry of purified RISCs 10 ng of AGO2 protein from each RISC, as quantified by titration filter binding, was denatured with 4X LDS sample buffer (Invitrogen, NP0007) and run on an SDS-polyacrylamide gel (NuPAGE 4 to 12%, Bis-Tris [Invitrogen, NP0322]) in MOPS buffer (Invitrogen, NP0001), according tomanufacturer’s instructions. .. Gels were stainedwith Imperial Blue Stain (Thermo Scientific, 24615).

    Titration:

    Article Title: The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.
    Article Snippet: .. Mass spectrometry of purified RISCs 10 ng of AGO2 protein from each RISC, as quantified by titration filter binding, was denatured with 4X LDS sample buffer (Invitrogen, NP0007) and run on an SDS-polyacrylamide gel (NuPAGE 4 to 12%, Bis-Tris [Invitrogen, NP0322]) in MOPS buffer (Invitrogen, NP0001), according tomanufacturer’s instructions. .. Gels were stainedwith Imperial Blue Stain (Thermo Scientific, 24615).

    Binding Assay:

    Article Title: The guide-RNA sequence dictates the slicing kinetics and conformational dynamics of the Argonaute silencing complex.
    Article Snippet: .. Mass spectrometry of purified RISCs 10 ng of AGO2 protein from each RISC, as quantified by titration filter binding, was denatured with 4X LDS sample buffer (Invitrogen, NP0007) and run on an SDS-polyacrylamide gel (NuPAGE 4 to 12%, Bis-Tris [Invitrogen, NP0322]) in MOPS buffer (Invitrogen, NP0001), according tomanufacturer’s instructions. .. Gels were stainedwith Imperial Blue Stain (Thermo Scientific, 24615).



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    Purification and biophysical characterization of EEPD1 . A , domain architecture of human EEPD1. B , Coomassie-stained SDS-PAGE gels showing the purified full-length EEPD1 and its nuclease domain, EEPD1 N uc (aa 261–569). C , chromatograms of the final Size-exclusion chromatography (SEC) chromatograms of full-length EEPD1 and EEPD1 N uc . A total of 3 mg of each protein was loaded onto the columns. D , SEC-MALS traces of EEPD1 and EEPD1nuc domain confirming that both proteins form a stable dimers in solution. E , SEC-MALS analysis demonstrating that EEPD1 dimerization is maintained in a reducing environment (2.0 mM DTT).

    Journal: The Journal of Biological Chemistry

    Article Title: Structural basis for the mechanism and stability of the EEPD1 5′ endonuclease

    doi: 10.1016/j.jbc.2026.111432

    Figure Lengend Snippet: Purification and biophysical characterization of EEPD1 . A , domain architecture of human EEPD1. B , Coomassie-stained SDS-PAGE gels showing the purified full-length EEPD1 and its nuclease domain, EEPD1 N uc (aa 261–569). C , chromatograms of the final Size-exclusion chromatography (SEC) chromatograms of full-length EEPD1 and EEPD1 N uc . A total of 3 mg of each protein was loaded onto the columns. D , SEC-MALS traces of EEPD1 and EEPD1nuc domain confirming that both proteins form a stable dimers in solution. E , SEC-MALS analysis demonstrating that EEPD1 dimerization is maintained in a reducing environment (2.0 mM DTT).

    Article Snippet: The isolated Flag-EEPD1 full length WT sample was loaded onto an SDS polyacrylamide gel (12% Mini-PROTEAN TGX, Bio-Rad Laboratories Inc) and electrophoreses to a distance of 1 cm and stained with Coomassie Brilliant Blue R-250.

    Techniques: Purification, Staining, SDS Page, Size-exclusion Chromatography